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  • 产品名称：InsulinGrowthFactorIReceptor(IGF-Ir)(AA31-932)(Active)protein(StrepTag,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
InsulinGrowthFactorIReceptor(IGF-Ir)(AA31-932)(Active)protein(StrepTag,Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 104 kDa (single chain), 81 kDa (α subunit) and 23 kDa (β subunit). The protein migrates as 120 kDa, 81 kDa and 35 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to bind human IGF-I in a functional ELISA.
Background The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60 % homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
Molecular Weight 104 kDa (single chain), 81 kDa (α subunit) and 23 kDa (β subunit)
UniProt P08069
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Insulin Growth Factor I Receptor (IGF-Ir) (AA 31-932) (Active) protein (Strep Tag,His tag) (ABIN2181260) Human IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Binding Studies (Bind) image for Insulin Growth Factor I Receptor (IGF-Ir) (AA 31-932) (Active) protein (Strep Tag,His tag) (ABIN2181260) Immobilized Human IGF-I, Fc Tag (Cat# IG1-H4269) at 5μg/mL (100 µl/well),can bind Hum...
Binding Studies (Bind) image for Insulin Growth Factor I Receptor (IGF-Ir) (AA 31-932) (Active) protein (Strep Tag,His tag) (ABIN2181260) Immobilized Human IGF-I R, His Tag (Cat# IGR-H5229) at 2μg/mL (100 µl/well),can bind ...
Background publications Jones, Clemmons: "Insulin-like growth factors and their binding proteins: biological actions." in: Endocrine reviews, Vol. 16, Issue 1, pp. 3-34, 1995 (PubMed).