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  • 产品名称：ActivinAReceptor,TypeIB(ACVR1B)(AA24-126)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ActivinAReceptor,TypeIB(ACVR1B)(AA24-126)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 38 kDa. As a result of glycosylation, the protein migrates as 43-46 kDa under reducing (R) condition, and 85-90 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 μg/mL ( 100 μL/well ) can bind rhACVR1b Fc Chimera with a linear range of 0.032 - 2 μg/mL
Background Activin receptor type-1B (ACVR1B) is also known as Activin receptor-like kinase 4 (ALK-4), Serine/threonine-protein kinase receptor R2 (SKR2), which belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. ACVR1B contains one GS domain and one protein kinase domain. The catalytic activity of ACVR1B is ""ATP + [receptor-protein] = ADP + [receptor-protein] phosphate"". ACVR1B acts as a transducer of activin or activin like ligands (e.g., inhibin) signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. These go on to recruit the R-SMADs SMAD2 or SMAD3. ACVR1B also transduces signals of nodal, GDF-1, and Vg1.
Molecular Weight 38 kDa
UniProt P36896
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Activin A Receptor, Type IB (ACVR1B) (AA 24-126) (Active) protein (Fc Tag) (ABIN2180556) Human Activin RIB, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditi...
Binding Studies (Bind) image for Activin A Receptor, Type IB (ACVR1B) (AA 24-126) (Active) protein (Fc Tag) (ABIN2180556) Immobilized Human Activin A, Tag Free with a linear range of 0.3-2.5 μg/mL.
Background publications Suzuki, Kobayashi, Funatsu, Morita, Ikekita: "Activin A induces neuronal differentiation and survival via ALK4 in a SMAD-independent manner in a subpopulation of human neuroblastomas." in: Biochemical and biophysical research communications, Vol. 394, Issue 3, pp. 639-45, 2010 (PubMed).

Harrison, Gray, Koerber, Fischer, Vale: "Identification of a functional for activin on the type I receptor ALK4." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 21129-35, 2003 (PubMed).

Cárcamo, Weis, Ventura, Wieser, Wrana, Attisano, Massagué: "Type I receptors specify growth-inhibitory and transcriptional responses to transforming growth factor beta and activin." in: Molecular and cellular biology, Vol. 14, Issue 6, pp. 3810-21, 1994 (PubMed).