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  • 产品名称：Glycerol-3-PhosphateDehydrogenase1(Soluble)(GPD1)(AA1-349)(Active)protein(Hista

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  • 产品厂商：ACROBiosystems
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简单介绍：
Glycerol-3-PhosphateDehydrogenase1(Soluble)(GPD1)(AA1-349)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 38.4 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic (GPD1), a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family, is also known as GPD-C or GPDH-C. The function of GPD1 is inhibited by zinc ions and sulfate. GPD1 can also interact selectively and non-covalently with nicotinamide adenine dinucleotide (NAD), a coenzyme involved in many redox and biosynthetic reactions, binding may be to either the oxidized form, NAD+, or the reduced form, NADH. Mutations in GPD1 gene are a cause of Hypertriglyceridemia, transient infantile (HTGIT).
Molecular Weight 38.4 kDa
UniProt P21695
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) (Active) protein (His tag) (ABIN2181184) Human GPD1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Basel-Vanagaite, Zevit, Har Zahav, Guo, Parathath, Pasmanik-Chor, McIntyre, Wang, Albin-Kaplanski, Hartman, Marom, Zeharia, Badir, Shoerman, Simon, Rechavi, Shohat, Hegele, Fisher, Shamir: "Transient infantile hypertriglyceridemia, fatty liver, and hepatic fibrosis caused by mutated GPD1, encoding glycerol-3-phosphate dehydrogenase 1." in: American journal of human genetics, Vol. 90, Issue 1, pp. 49-60, 2012 (PubMed).

Ou, Ji, Han, Zhao, Li, Mao, Wong, Bartlam, Rao: "Crystal structures of human glycerol 3-phosphate dehydrogenase 1 (GPD1)." in: Journal of molecular biology, Vol. 357, Issue 3, pp. 858-69, 2006 (PubMed).