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  • 产品名称：AnnexinA5(ANXA5)(AA1-320)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
AnnexinA5(ANXA5)(AA1-320)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.8 kDa. The protein migrates as 36.8 kDa under reducing (R) condition (SDS-PAGE).
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Anexin A5 is a phospholipid binding protein, which binds with high affinity and selectivity to PS in the presence of calcium. PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Anexin A5 will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Anexin A5 has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Anexin A5 is associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
Molecular Weight 36.8 kDa
UniProt P08758
Pathways Apoptosis
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Annexin A5 (ANXA5) (AA 1-320) (Active) protein (His tag) (ABIN2180594) Annexin A5 (ANXA5) (AA 1-320) (Active) protein (His tag)
Background publications Elegbede, Srivastava, Hinderliter: "Purification of recombinant annexins without the use of phospholipids." in: Protein expression and purification, Vol. 50, Issue 2, pp. 157-62, 2006 (PubMed).

Brachvogel, Welzel, Moch, von der Mark, Hofmann, Pöschl: "Sequential expression of annexin A5 in the vasculature and skeletal elements during mouse development." in: Mechanisms of development, Vol. 109, Issue 2, pp. 389-93, 2001 (PubMed).