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  • 产品名称：Interferongamma(IFNG)(AA24-166)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interferongamma(IFNG)(AA24-166)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 16.8 kDa. The protein migrates as 17 kDa,20 kDa and 24 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Determined by a cytotoxicity assay using HT-29 cells. The ED50 < 0.05 ng/mL, corresponding to a specific activity of >2X107 Unit/mg.
Background Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
Molecular Weight 16.8 kDa
UniProt P01579
Research Area Cytokines
Pathways Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Interferon gamma (IFNG) (AA 24-166) (Active) Protein (ABIN2181249) Human IFN-gamma on SDS-PAGE under reducing (R) condition. The gel was stained overnig...
Background publications Miller, Maher, Young: "Clinical Use of Interferon-gamma." in: Annals of the New York Academy of Sciences, Vol. 1182, pp. 69-79, 2010 (PubMed).

Derynck, Leung, Gray, Goeddel: "Human interferon gamma is encoded by a single class of mRNA." in: Nucleic acids research, Vol. 10, Issue 12, pp. 3605-15, 1982 (PubMed).