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  • 产品名称：ProteinA(AA2-293)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProteinA(AA2-293)(Active)protein(Histag)
详情介绍：
Brand MABSol®
Characteristics Recombinant Protein A Protein is fused with the polyhistidine tag at the N-terminus consisting 299 amino acids. The reducing (R) protein migrates as a 33.8 kDa polypeptide .Protein A Protein can bind to human IgG1 Fc.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody . Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing.
UniProt P02976
Research Area Coagulation
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Protein A (AA 2-293) (Active) protein (His tag) (ABIN2181667) Protein A (AA 2-293) (Active) protein (His tag)
Background publications Hartleib, Köhler, Dickinson, Chhatwal, Sixma, Hartford, Foster, Peters, Kehrel, Herrmann: "Protein A is the von Willebrand factor binding protein on Staphylococcus aureus." in: Blood, Vol. 96, Issue 6, pp. 2149-56, 2000 (PubMed).

Kristiansen, Pascual, Lipsky: "Staphylococcal protein A induces biased production of Ig by VH3-expressing B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 7, pp. 2974-82, 1994 (PubMed).

Romagnani, Giudizi, Almerigogna, Biagiotti, Bellesi, Bernardi, Ricci: "Protein A reactivity of IgM- and IgD-bearing lymphocytes from some patients with chronic lymphocytic leukemia." in: Clinical immunology and immunopathology, Vol. 19, Issue 1, pp. 139-48, 1981 (PubMed).

Romagnani, Giudizi, Biagiotti, Almerigogna, Maggi, Del Prete, Ricci: "Surface immunoglobulins are involved in the interaction of protein A with human B cells and in the triggering of B cell proliferation induced by protein A-containing Staphylococcus aureus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 4, pp. 1307-13, 1981 (PubMed).