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  • 产品名称：CystatinSN(CST1)(AA21-141)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CystatinSN(CST1)(AA21-141)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.1 kDa. The protein migrates as 17-19 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Cystatin-1 (CST1) is also known as Cystatin-SN, Cystain-SA-I, Salivary cystatin-SA-1, which is secreted protein and belongs to the cystatin family. Cystatin-1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
Molecular Weight 15.1 kDa
UniProt P01037
Research Area Proteolysis / Ubiquitin
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 25 mM MES, 150 mM NaCl, pH 6.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cystatin SN (CST1) (AA 21-141) (Active) protein (His tag) (ABIN2180915) Human Cystatin SN, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
Background publications Ryan, Souda, Halgand, Wong, Loo, Faull, Whitelegge: "Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry." in: Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 6, pp. 908-17, 2010 (PubMed).

Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004 (PubMed).