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  • 产品名称：VascularEndothelialGrowthFactor(VEGF)(AA27-190)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
VascularEndothelialGrowthFactor(VEGF)(AA27-190)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade HPLC verified
ProductDetails: Biological Activity Comment Biological Activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells ( HUVEC ). The ED50 for this effect is typically 3.5 ng/mL.

SDS-PAGE: due to different glycosylation.
Background Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
Molecular Weight 19.3 kDa
UniProt Q00731
Research Area Signaling, Receptors, Immunology, Cytokines, Growth Factors, Inflammation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-190) (Active) Protein (ABIN2181901) Mouse VEGF164 on SDS-PAGE under reducing (R) condition. The gel was stained overnight...
Binding Studies (Bind) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-190) (Active) Protein (ABIN2181901) Immobilized Mouse VEGF164 (Cat# VE4-M4216) at 2 μg/mL (100 μL/well) can bind VEGFR2 ...
Background publications Shin, Lee, Ko, Lee, Park: "The regulators of VEGF expression in mouse ovaries." in: Yonsei medical journal, Vol. 46, Issue 5, pp. 679-86, 2005 (PubMed).

Breier, Albrecht, Sterrer, Risau: "Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation." in: Development (Cambridge, England), Vol. 114, Issue 2, pp. 521-32, 1992 (PubMed).