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  • 产品名称：Kallikrein1-RelatedPeptidaseB22(KLK1B22)(AA18-259)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Kallikrein1-RelatedPeptidaseB22(KLK1B22)(AA18-259)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.3 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Kallikrein 1-related peptidase b22 (Klk1b22) is also known as Beta-NGF-endopeptidase, Epidermal growth factor-binding protein type A (EGF-BP A), Glandular kallikrein K22 (mGK-22), Nerve growth factor beta chain endopeptidase, Tissue kallikrein 22 (KLK22), which belongs to the peptidase S1 family and Kallikrein subfamily. Klk1b22 contains one peptidase S1 domain. Klk1b22 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Molecular Weight 27.3 kDa
NCBI Accession NP_034244
UniProt P15948
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5 with 0.05 % CHAPS
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Kallikrein 1-Related Peptidase B22 (KLK1B22) (AA 18-259) (Active) protein (His tag) (ABIN2181434) Mouse Kallikrein 22, His Tag on SDS-PAGE under reducing (R) condition. The gel was st...
Background publications Drinkwater, Evans, Richards: "Mouse glandular kallikrein genes: identification and characterization of the genes encoding the epidermal growth factor binding proteins." in: Biochemistry, Vol. 26, Issue 21, pp. 6750-6, 1988 (PubMed).

Evans, Drinkwater, Richards: "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." in: The Journal of biological chemistry, Vol. 262, Issue 17, pp. 8027-34, 1987 (PubMed).