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  • 产品名称：CD4Molecule(CD4)(AA26-390)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD4Molecule(CD4)(AA26-390)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 41.5 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When 50000 cells/well are added to human recombinant CD4 coated plates (2.5 μg/mL, 100 μL/well), approximately 50 - 80 % of the cells will adhere after 1 hour at 37°C.

SDS-PAGE: due to glycosylation.
Background T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
Molecular Weight 41.5 kDa
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD4 Molecule (CD4) (AA 26-390) (Active) protein (His tag) (ABIN2180791) Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Zeitlmann, Sirim, Kremmer, Kolanus: "Cloning of ACP33 as a novel intracellular ligand of CD4." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9123-32, 2001 (PubMed).

Lusso, Secchiero, Crowley, Garzino-Demo, Berneman, Gallo: "CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 9, pp. 3872-6, 1994 (PubMed).

Crise, Buonocore, Rose: "CD4 is retained in the endoplasmic reticulum by the human immunodeficiency virus type 1 glycoprotein precursor." in: Journal of virology, Vol. 64, Issue 11, pp. 5585-93, 1990 (PubMed).