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  • 产品名称：CD40Molecule,TNFReceptorSuperfamilyMember5(CD40)(AA21-193)(Active)protein(Hista

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD40Molecule,TNFReceptorSuperfamilyMember5(CD40)(AA21-193)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD40/TNFRSF5 at 1 μg/mL (100 μL/well) can bind Recombinant Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.
Background Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).
Molecular Weight 20 kDa
UniProt P25942
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 21-193) (Active) protein (His tag) (ABIN2180793) Human CD40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Binding Studies (Bind) image for CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 21-193) (Active) protein (His tag) (ABIN2180793) Immobilized Human CD40, His Tag with a linear range of 2.6-16 ng/mL.
Background publications Ferrari, Giliani, Insalaco, Al-Ghonaium, Soresina, Loubser, Avanzini, Marconi, Badolato, Ugazio, Levy, Catalan, Durandy, Tbakhi, Notarangelo, Plebani: "Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 22, pp. 12614-9, 2001 (PubMed).