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  • 产品名称：Kallikrein1(KLK1)(AA19-262)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Kallikrein1(KLK1)(AA19-262)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave a flourogenic peptide substrate Pro - Phe - Arg - 7 - amido - 4 - methylcoumarin (PFR-AMC). The specific activity is >1200 pmol/min/μg.
Background Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Molecular Weight 27.9 kDa
NCBI Accession NP_002248
UniProt P06870
Pathways Complement System
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Kallikrein 1 (KLK1) (AA 19-262) (Active) protein (His tag) (ABIN2181428) Human Kallikrein 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was sta...
Background publications Chen, Murray, Chai, Chao, Chao: "Molecular cloning and characterization of a novel kallikrein transcript in colon and its distribution in human tissues." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], Vol. 27, Issue 8, pp. 1829-38, 1995 (PubMed).

Fisman, Pines, Ben-Ari, Shiner, Drory, Friedman, Kellermann: "Echocardiographic evaluation of the effects of gallopamil on left ventricular function." in: Clinical pharmacology and therapeutics, Vol. 44, Issue 1, pp. 100-6, 1988 (PubMed).

Kellermann, Lottspeich, Geiger, Deutzmann: "Human urinary kallikrein--amino acid sequence and carbohydrate attachment sites." in: Protein sequences & data analysis, Vol. 1, Issue 3, pp. 177-82, 1988 (PubMed).