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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member10a(TNFRSF10A)(AA24-239)(Active)pr

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member10a(TNFRSF10A)(AA24-239)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24 kDa. The protein migrates as 22-24 kDa under reducing (R) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily. TRAILR1 / TNFRSF10A contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10A / DR4 is widely expressed and high levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. APO2 / TNFRSF10A is receptor for the cytotoxic ligand TNFSF10 / TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAILR-1 / DR4 / CD261 promotes the activation of NF-kappa-B.
Molecular Weight 24 kDa
NCBI Accession NP_003835
UniProt O00220
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A) (AA 24-239) (Active) protein (His tag) (ABIN2181862) Human TRAIL R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained...
Background publications Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, 2008 (PubMed).

Doubravská, Símová, Cermak, Valenta, Korínek, Andera: "Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 4, pp. 573-87, 2008 (PubMed).

Chaudhary, Eby, Jasmin, Bookwalter, Murray, Hood: "Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway." in: Immunity, Vol. 7, Issue 6, pp. 821-30, 1998 (PubMed).