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  • 产品名称：Interleukin1Receptor,TypeII(IL1R2)(AA14-343)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interleukin1Receptor,TypeII(IL1R2)(AA14-343)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.6 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 μg/mL of IL1R2 will inhibit 50% of the biological response due to 50 pg/mL of recombinant human IL-1 beta.
Background Interleukin-1 receptor type 2 (IL1R2) is also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity, this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Molecular Weight 38.6 kDa
NCBI Accession NP_004624
UniProt P27930
Pathways NF-kappaB Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Interleukin 1 Receptor, Type II (IL1R2) (AA 14-343) (Active) protein (His tag) (ABIN2181357) Interleukin 1 Receptor, Type II (IL1R2) (AA 14-343) (Active) protein (His tag)
Background publications Wang, Zhang, Li, Liu, Mei, Wang: "Structural insights into the assembly and activation of IL-1? with its receptors." in: Nature immunology, Vol. 11, Issue 10, pp. 905-11, 2010 (PubMed).

Orlando, Sironi, Bianchi, Drummond, Boraschi, Yabes, Mantovani: "Role of metalloproteases in the release of the IL-1 type II decoy receptor." in: The Journal of biological chemistry, Vol. 272, Issue 50, pp. 31764-9, 1998 (PubMed).

Orlando, Matteucci, Fadlon, Buurman, Bardella, Colotta, Introna, Mantovani: "TNF-alpha, unlike other pro- and anti-inflammatory cytokines, induces rapid release of the IL-1 type II decoy receptor in human myelomonocytic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 8, pp. 3861-8, 1997 (PubMed).

Liu, Hart, Liu, Clevenger, Maki, De Souza: "Cloning and characterization of an alternatively processed human type II interleukin-1 receptor mRNA." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20965-72, 1996 (PubMed).

Giri, Wells, Dower, McCall, Guzman, Slack, Bird, Shanebeck, Grabstein, Sims: "Elevated levels of shed type II IL-1 receptor in sepsis. Potential role for type II receptor in regulation of IL-1 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 12, pp. 5802-9, 1995 (PubMed).

Ploski, McDowell, Symons, Flatø, Duff, Thorsby, Førre: "Interaction between HLA-DR and HLA-DP, and between HLA and interleukin 1 alpha in juvenile rheumatoid arthritis indicates heterogeneity of pathogenic mechanisms of the disease." in: Human immunology, Vol. 42, Issue 4, pp. 343-7, 1995 (PubMed).

Symons, Young, Duff: "Soluble type II interleukin 1 (IL-1) receptor binds and blocks processing of IL-1 beta precursor and loses affinity for IL-1 receptor antagonist." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 5, pp. 1714-8, 1995 (PubMed).