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  • 产品名称：BCL2-Like1(BCL2L1)(AA1-212)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
BCL2-Like1(BCL2L1)(AA1-212)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.6 kDa. The protein migrates as 26 kDa under reducing (R) condition (SDS-PAGE).
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human BID protein at 10 μg/mL (100 μL/well) can bind biotinylated human BCL2L1 protein, The EC50 of biotinylated human BCL2L1 is 6.5 ng/mL.
Background Bcl-2-like protein 1 (BCL2L1), is also known as Apoptosis regulator Bcl-X (Bcl-XL). BCL2L1 / Bcl-XL is a homodimer protein and belongs to the Bcl-2 family. BCL2L1 is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. BCL2L1 is a potent inhibitor of cell death. BCL2L1 Inhibits activation of caspases. BCL2L1 appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. BCL2L1 / Bcl-XL also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.
Molecular Weight 24.6 kDa
UniProt Q07817
Research Area Cancer
Pathways Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM HEPES, 50 mM KCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for BCL2-Like 1 (BCL2L1) (AA 1-212) (Active) protein (His tag) (ABIN2180638) Human Bcl-x, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Terrano, Upreti, Chambers: "Cyclin-dependent kinase 1-mediated Bcl-xL/Bcl-2 phosphorylation acts as a functional link coupling mitotic arrest and apoptosis." in: Molecular and cellular biology, Vol. 30, Issue 3, pp. 640-56, 2010 (PubMed).