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  • 产品名称：CD274Molecule(CD274)(AA19-238)(Active)protein(StrepTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD274Molecule(CD274)(AA19-238)(Active)protein(StrepTag)
详情介绍：
Characteristics This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Molecular Weight 28.2 kDa
NCBI Accession NP_054862
UniProt Q9NZQ7
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (Strep Tag) (ABIN2181641) Human PD-L1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Binding Studies (Bind) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (Strep Tag) (ABIN2181641) Immobilized Human PD-L1, Strep Tag with a linear range of 2-60 ng/mL.
Background publications Freeman, Long, Iwai, Bourque, Chernova, Nishimura, Fitz, Malenkovich, Okazaki, Byrne, Horton, Fouser, Carter, Ling, Bowman, Carreno, Collins, Wood, Honjo: "Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1027-34, 2000 (PubMed).

Dong, Zhu, Tamada, Chen: "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion." in: Nature medicine, Vol. 5, Issue 12, pp. 1365-9, 1999 (PubMed).