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  • 产品名称：DickkopfHomolog1(XenopusLaevis)(DKK1)(AA32-266)(Active)protein(StrepTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
DickkopfHomolog1(XenopusLaevis)(DKK1)(AA32-266)(Active)protein(StrepTag)
详情介绍：
Characteristics This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 28.8 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.
Molecular Weight 28.8 kDa
NCBI Accession NP_036374
UniProt O94907
Pathways WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) (AA 32-266) (Active) protein (Strep Tag) (ABIN2180963) Human Dkk-1, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Background publications Li, Pazgier, Li, Li, Liu, Zou, Li, Chen, Tarasov, Lu, Lu: "Limitations of peptide retro-inverso isomerization in molecular mimicry." in: The Journal of biological chemistry, Vol. 285, Issue 25, pp. 19572-81, 2010 (PubMed).