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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member14(TNFRSF14)(AA39-202)(Active)prot

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member14(TNFRSF14)(AA39-202)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.4 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2.5-10 μg/mL in the presence of 1 ng/mL of recombinant human TNF-beta.

SDS-PAGE: resulting from glycosylation.
Background Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.
Molecular Weight 45.4 kDa
NCBI Accession NP_003811
UniProt Q92956
Pathways Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) (Active) protein (Fc Tag) (ABIN2181236) Human HVEM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Binding Studies (Bind) image for Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) (Active) protein (Fc Tag) (ABIN2181236) Immobilized Human BTLA, His Tag (Cat # BTA-H52E0) at 5 μg/mL (100 μL/well) can bind H...
Background publications Cheung, Humphreys, Potter, Norris, Shumway, Tran, Patterson, Jean-Jacques, Yoon, Spear, Murphy, Lurain, Benedict, Ware: "Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 37, pp. 13218-23, 2005 (PubMed).