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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member1A(TNFRSF1A)(AA22-211)(Active)prot

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member1A(TNFRSF1A)(AA22-211)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human TNFa (Catalog # TNA-H4211) at 5 μg/mL (100 μL/well) can bind human TNFR1 / CD120a (Catalog # TN1-H5222) . The EC50 of human TNFR1 is 510 ng/mL.

SDS-PAGE: due to different glycosylation.
Background Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).
Molecular Weight 22 kDa
NCBI Accession NP_001056
UniProt P19438
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (AA 22-211) (Active) protein (His tag) (ABIN2181837) Human TNFR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Gregory, Dendrou, Attfield, Haghikia, Xifara, Butter, Poschmann, Kaur, Lambert, Leach, Prömel, Punwani, Felce, Davis, Gold, Nielsen, Siegel, Mann, Bell, McVean, Fugger: "TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis." in: Nature, Vol. 488, Issue 7412, pp. 508-11, 2012 (PubMed).

Le, Trilling, Hengel: "The cytomegaloviral protein pUL138 acts as potentiator of tumor necrosis factor (TNF) receptor 1 surface density to enhance ULb'-encoded modulation of TNF-? signaling." in: Journal of virology, Vol. 85, Issue 24, pp. 13260-70, 2011 (PubMed).

Jiang, Woronicz, Liu, Goeddel: "Prevention of constitutive TNF receptor 1 signaling by silencer of death domains." in: Science (New York, N.Y.), Vol. 283, Issue 5401, pp. 543-6, 1999 (PubMed).