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  • 产品名称：SulfotransferaseFamily,Cytosolic,2B,Member1(SULT2B1)(AA1-365)(Active)protein(Hi

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  • 产品厂商：ACROBiosystems
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简单介绍：
SulfotransferaseFamily,Cytosolic,2B,Member1(SULT2B1)(AA1-365)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 42.1 kDa. The protein migrates as 46 kDa under reducing (R) condition (SDS-PAGE).
Purity >80 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Sulfotransferase family cytosolic 2B member 1 (SULT2B1),is also known as sulfotransferase 2B1 (ST2B1), alcohol sulfotransferase and hydroxysteroid sulfotransferase 2 (HSST2), which is expressed highly in placenta, prostate and trachea and lower expression in the small intestine and lung. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites.
Molecular Weight 42.1 kDa
UniProt O00204
Research Area Signaling, Metabolism
Pathways Steroid Hormone Biosynthesis
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 8.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Sulfotransferase Family, Cytosolic, 2B, Member 1 (SULT2B1) (AA 1-365) (Active) protein (His tag) (ABIN2181796) Human SULT2B1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...
Background publications Salman, He, Runge-Morris, Kocarek, Falany: "Site-directed mutagenesis of human cytosolic sulfotransferase (SULT) 2B1b to phospho-mimetic Ser348Asp results in an isoform with increased catalytic activity." in: The Journal of steroid biochemistry and molecular biology, Vol. 127, Issue 3-5, pp. 315-23, 2011 (PubMed).

Lee, Fuda, Lee, Negishi, Strott, Pedersen et al.: "Crystal structure of human cholesterol sulfotransferase (SULT2B1b) in the presence of pregnenolone and 3'-phosphoadenosine 5'-phosphate. Rationale for specificity differences between prototypical ..." in: The Journal of biological chemistry, Vol. 278, Issue 45, pp. 44593-9, 2003 (PubMed).

Fuda, Lee, Shimizu, Javitt, Strott et al.: "Mutational analysis of human hydroxysteroid sulfotransferase SULT2B1 isoforms reveals that exon 1B of the SULT2B1 gene produces cholesterol sulfotransferase, whereas exon 1A yields pregnenolone ..." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 36161-6, 2002 (PubMed).