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  • 产品名称：VascularCellAdhesionMolecule1(VCAM1)(AA25-697)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
VascularCellAdhesionMolecule1(VCAM1)(AA25-697)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 75 kDa. The protein migrates as 80-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 μg/mL with 100 μL/well), >75% cells will adhere after 1 hour incubation at 37°C. Optimal dilutions should be determined by each laboratory for each application.
Background Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. VCAM1 / CD106 contains 7 Ig-like C2-type (immunoglobulin-like) domains. CD106 / VCAM-1 is expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. L1CAM / VCAM-1 is Important in cell-cell recognition and appears to function in leukocyte-endothelial cell adhesion. CD106 / VCAM1 interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1 / VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. INCAM-100 / VCAM1 binds to ECMV-D capsid proteins and acts as a receptor for this virus.
Molecular Weight 75 kDa
NCBI Accession NP_001069
UniProt P19320
Pathways Carbohydrate Homeostasis
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-697) (Active) protein (His tag) (ABIN2181894) Human VCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004 (PubMed).

Osborn, Vassallo, Benjamin: "Activated endothelium binds lymphocytes through a novel in the alternately spliced domain of vascular cell adhesion molecule-1." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 99-107, 1992 (PubMed).