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  • 产品名称：PoliovirusReceptor-Related1(HerpesvirusEntryMediatorC)(PVRL1)(AA31-334)(Active)

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  • 产品厂商：ACROBiosystems
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简单介绍：
PoliovirusReceptor-Related1(HerpesvirusEntryMediatorC)(PVRL1)(AA31-334)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.8 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Nectin-3 /PVRL3 at 1 μg/mL (100 μL/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.25-1000 ng/mL.
Background Poliovirus receptor-related protein 1 (PVRL1) is also known as Herpes virus entry mediator C (HveC), Herpesvirus Ig-like receptor (HIgR), Nectin-1, CD antigen CD111, PVRL1 / Nectin-1 / CD111 belongs to the nectin family. PVRL1 / Nectin-1 is a membrane protein with three extracellular immunoglobulin domains, a single transmembrane helix and a cytoplasmic tail. The protein can mediate Ca2+-independent cellular adhesion further characterizing it as IgSF cell adhesion molecule (IgSF CAM). PVRL1 is adhesion molecule found in a wide range of tissues. PVRL1 / Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.
Molecular Weight 34.8 kDa
NCBI Accession NP_002846
UniProt Q15223
Pathways Cell-Cell Junction Organization
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1) (AA 31-334) (Active) protein (His tag) (ABIN2181679) Human Nectin-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained...
Background publications Suzuki, Hu, Bustos, Zlotogora, Richieri-Costa, Helms, Spritz: "Mutations of PVRL1, encoding a cell-cell adhesion molecule/herpesvirus receptor, in cleft lip/palate-ectodermal dysplasia." in: Nature genetics, Vol. 25, Issue 4, pp. 427-30, 2000 (PubMed).

Lopez, Eberlé, Mattei, Gabert, Birg, Bardin, Maroc, Dubreuil: "Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene." in: Gene, Vol. 155, Issue 2, pp. 261-5, 1995 (PubMed).