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  • 产品名称：CystatinS(CST4)(AA21-141)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CystatinS(CST4)(AA21-141)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is <1.0 μm.="" <="" td="">
Background Cystatin-4 (CST4) is also known as Cystatin-S, Salivary acidic protein 1, which is a secreted protein, which belongs to the cystatin family. Cystatin-S is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) and is also expressed in saliva, tears, urine and seminal fluid. Cystatin-4 / Cystatin S strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.
Molecular Weight 15 kDa
UniProt P01036
Research Area Proteolysis / Ubiquitin
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 25 mM MES, 150 mM NaCl, pH 6.0
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cystatin S (CST4) (AA 21-141) (Active) protein (His tag) (ABIN2180919) Human Cystatin S, His Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Background publications Tanay, Lotan, Bernstein: "Salutary proximal processes and distal mood and anxiety vulnerability outcomes of mindfulness training: a pilot preventive intervention." in: Behavior therapy, Vol. 43, Issue 3, pp. 492-505, 2012 (PubMed).

Ryan, Souda, Halgand, Wong, Loo, Faull, Whitelegge: "Confident assignment of intact mass tags to human salivary cystatins using top-down Fourier-transform ion cyclotron resonance mass spectrometry." in: Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 6, pp. 908-17, 2010 (PubMed).

Isemura, Saitoh, Sanada, Minakata et al.: "Identification of full-sized forms of salivary (S-type) cystatins (cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms of cystatin S) in human whole saliva and determination of ..." in: Journal of biochemistry, Vol. 110, Issue 4, pp. 648-54, 1992 (PubMed).

Lamkin, Jensen, Setayesh, Troxler, Oppenheim: "Salivary cystatin SA-III, a potential precursor of the acquired enamel pellicle, is phosphorylated at both its amino- and carboxyl-terminal regions." in: Archives of biochemistry and biophysics, Vol. 288, Issue 2, pp. 664-70, 1991 (PubMed).