产品详情
查看大图

  • 产品名称：CartilageOligomericMatrixProtein(COMP)(AA21-757)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CartilageOligomericMatrixProtein(COMP)(AA21-757)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 82 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >88 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells, when rhCOMP / Thrombospondin-5 immobilized at 1 μg/well. More than 45% of ATDC-5 cell adhesion will be induced.

SDS-PAGE: due to glycosylation.
Background Cartilage oligomeric matrix protein (COMP) is also known as Thrombospondin-5 (TSP5), EDM1, EPD1, MED, PSACH, THBS5, which belongs to the thrombospondin family. COMP / TSP5 contains 4 EGF-like domains, 1 TSP C-terminal (TSPC) domain, 8 TSP type-3 repeats. Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. COMP may play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. COMP can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Thrombospondin-5 could play a role in the pathogenesis of osteoarthritis. COMP is a marker of cartilage turnover.
Molecular Weight 82 kDa
UniProt P49747
Research Area Stem Cells, Extracellular Matrix
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Cartilage Oligomeric Matrix Protein (COMP) (AA 21-757) (Active) protein (His tag) (ABIN2180897) Human COMP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Gagarina, Carlberg, Pereira-Mouries, Hall: "Cartilage oligomeric matrix protein protects cells against death by elevating members of the IAP family of survival proteins." in: The Journal of biological chemistry, Vol. 283, Issue 1, pp. 648-59, 2007 (PubMed).

Chen, Thomas, Hecht, Goldring, Lawler: "Cartilage oligomeric matrix protein/thrombospondin 5 supports chondrocyte attachment through interaction with integrins." in: The Journal of biological chemistry, Vol. 280, Issue 38, pp. 32655-61, 2005 (PubMed).