产品详情
查看大图

  • 产品名称：CD84Molecule(CD84)(AA22-225)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CD84Molecule(CD84)(AA22-225)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured in a cell proliferation assay using PHA stimulated human T cells in the presence of anti-CD3. The ED50 for this effect is typically 2-6 μg/mL in the presence of anti-CD3 immobilized at 20 ng/mL.
Background Leukocyte differentiation antigen CD84 is also known as SLAM family member 5 (SLAMF5), which belongs to immunoglobulin (Ig) superfamily. CD84 / SLAMF5 contains one Ig-like C2-type (immunoglobulin-like) domain. CD84 plays a role as adhesion receptor functioning by homophilic interactions and by clustering. CD84 / SLAMF5 increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway.
Molecular Weight 23.6 kDa
UniProt Q9UIB8
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD84 Molecule (CD84) (AA 22-225) (Active) protein (His tag) (ABIN2180849) Human CD84, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Zaiss, Hirtreiter, Rehli, Rehm, Kunz-Schughart, Andreesen, Hennemann: "CD84 expression on human hematopoietic progenitor cells." in: Experimental hematology, Vol. 31, Issue 9, pp. 798-805, 2003 (PubMed).

Tangye, van de Weerdt, Avery, Hodgkin: "CD84 is up-regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT-2." in: European journal of immunology, Vol. 32, Issue 6, pp. 1640-9, 2002 (PubMed).

Martin, Romero, de la Fuente, Tovar, Zapater, Esplugues, Pizcueta, Bosch, Engel: "CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 7, pp. 3668-76, 2001 (PubMed).