产品详情
查看大图

  • 产品名称：CD68Molecule(CD68)(AA22-319)(Active)protein(FcTag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CD68Molecule(CD68)(AA22-319)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 59.2 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background CD antigen CD68 is also known as Macrosialin, Gp110, which belongs to the LAMP family. CD68 / Macrosialin is highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 / Macrosialin could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 / Macrosialin binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Molecular Weight 59.2 kDa
UniProt P34810
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD68 Molecule (CD68) (AA 22-319) (Active) protein (Fc Tag) (ABIN2180823) Human CD68, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Gottfried, Kreutz, Mackensen: "Tumor-induced modulation of dendritic cell function." in: Cytokine & growth factor reviews, Vol. 19, Issue 1, pp. 65-77, 2008 (PubMed).

Gottfried, Kunz-Schughart, Weber, Rehli, Peuker, Müller, Kastenberger, Brockhoff, Andreesen, Kreutz: "Expression of CD68 in non-myeloid cell types." in: Scandinavian journal of immunology, Vol. 67, Issue 5, pp. 453-63, 2008 (PubMed).

Beranek: "CD68 is not a macrophage-specific antigen." in: Annals of the rheumatic diseases, Vol. 64, Issue 2, pp. 342-3; author reply 343-4, 2005 (PubMed).

Kunisch, Fuhrmann, Roth, Winter, Lungershausen, Kinne: "Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry." in: Annals of the rheumatic diseases, Vol. 63, Issue 7, pp. 774-84, 2004 (PubMed).