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  • 产品名称：V-SetDomainContainingTCellActivationInhibitor1(VTCN1)(AA29-258)(Active)protein(

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-SetDomainContainingTCellActivationInhibitor1(VTCN1)(AA29-258)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.75 - 3 μg/mL.
Background V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
Molecular Weight 26.1 kDa
NCBI Accession NP_078902
UniProt Q7Z7D3
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (His tag) (ABIN2180624) Human B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Binding Studies (Bind) image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (His tag) (ABIN2180624) Immobilized Human B7-H4, His Tag with a linear range of 0.4-6 ng/mL.
Background publications Miyatake, Tringler, Liu, Liu, Papkoff, Enomoto, Torkko, Dehn, Swisher, Shroyer: "B7-H4 (DD-O110) is overexpressed in high risk uterine endometrioid adenocarcinomas and inversely correlated with tumor T-cell infiltration." in: Gynecologic oncology, Vol. 106, Issue 1, pp. 119-27, 2007 (PubMed).

Wei, Kryczek, Zou: "Regulatory T-cell compartmentalization and trafficking." in: Blood, Vol. 108, Issue 2, pp. 426-31, 2006 (PubMed).

Kryczek, Zou, Rodriguez, Zhu, Wei, Mottram, Brumlik, Cheng, Curiel, Myers, Lackner, Alvarez, Ochoa, Chen, Zou: "B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma." in: The Journal of experimental medicine, Vol. 203, Issue 4, pp. 871-81, 2006 (PubMed).

Salceda, Tang, Kmet, Munteanu, Ghosh, Macina, Liu, Pilkington, Papkoff: "The immunomodulatory protein B7-H4 is overexpressed in breast and ovarian cancers and promotes epithelial cell transformation." in: Experimental cell research, Vol. 306, Issue 1, pp. 128-41, 2005 (PubMed).