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  • 产品名称：EphrinB1(EFNB1)(AA28-232)(Active)protein(StrepTag,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EphrinB1(EFNB1)(AA28-232)(Active)protein(StrepTag,Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.2 kDa. The protein migrates as 32-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human EphrinB1 at 10 μg/mL (100 μL/well) can bind human EphB6 with a linear range of 0.2-8 μg/mL.
Background Ephrin-B1 (EFNB1) is also known as EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2 (LERK-2), which belongs to the ephrin family. EFNB1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNB1 binds to the receptor tyrosine kinases EPHB1 and EPHA1. EFNB1 binds to, and induce the collapse of, commissural axons/growth cones in vitro. EFNB1 may play a role in constraining the orientation of longitudinally projecting axons. EFNB1 interacts with GRIP1 and GRIP2.
Molecular Weight 23.2 kDa
NCBI Accession NP_004420
UniProt P98172
Pathways RTK Signaling
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Ephrin B1 (EFNB1) (AA 28-232) (Active) protein (Strep Tag,His tag) (ABIN2180995) Human Ephrin-B1, His Tag on SDS-PAGE under reducing (R) condition. The gel was staine...
Background publications Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004 (PubMed).

Al-Batran, Astner, Supthut, Gamarra, Brueckner, Welsch, Knuechel, Huber: "Three-dimensional in vitro cocultivation of lung carcinoma cells with human bronchial organ culture as a model for bronchial carcinoma." in: American journal of respiratory cell and molecular biology, Vol. 21, Issue 2, pp. 200-8, 1999 (PubMed).

Brückner, Pablo Labrador, Scheiffele, Herb, Seeburg, Klein: "EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomains." in: Neuron, Vol. 22, Issue 3, pp. 511-24, 1999 (PubMed).