产品详情
查看大图

  • 产品名称：CoxsackieVirusandAdenovirusReceptor(CXADR)(AA20-237)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CoxsackieVirusandAdenovirusReceptor(CXADR)(AA20-237)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.9 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 50000 cells/well are added to CXADR coated plates ( 4 μg/mL and 100 μL/well ), approximately 20% - 50% will adhere specifically after 60 minutes at 37°C.

SDS-PAGE: due to glycosylation.
Background Coxsackie virus and adenovirus receptor (CXADR) is also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V- type (D1) and a C2- type (D2) Ig-like domain, a transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.
Molecular Weight 24.9 kDa
NCBI Accession NP_001329
UniProt P78310
Pathways Cell-Cell Junction Organization
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Coxsackie Virus and Adenovirus Receptor (CXADR) (AA 20-237) (Active) protein (His tag) (ABIN2180951) Human CXADR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Raschperger, Thyberg, Pettersson, Philipson, Fuxe, Pettersson: "The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis." in: Experimental cell research, Vol. 312, Issue 9, pp. 1566-80, 2006 (PubMed).

Bergelson, Cunningham, Droguett, Kurt-Jones, Krithivas, Hong, Horwitz, Crowell, Finberg: "Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5." in: Science (New York, N.Y.), Vol. 275, Issue 5304, pp. 1320-3, 1997 (PubMed).