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  • 产品名称：CD200Molecule(CD200)(AA31-232)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD200Molecule(CD200)(AA31-232)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 48.6 kDa. The protein migrates as 50-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 μg/mL,the concentration of rhCD200 Fc Chimera that produces 50% of the optimal binding response is appoximately 2.0-16 ng/mL.
Background CD200 is also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
Molecular Weight 48.6 kDa
UniProt P41217
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD200 Molecule (CD200) (AA 31-232) (Active) protein (Fc Tag) (ABIN2180724) Human CD200, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications McCaughan, Clark, Barclay: "Characterization of the human homolog of the rat MRC OX-2 membrane glycoprotein." in: Immunogenetics, Vol. 25, Issue 5, pp. 329-35, 1987 (PubMed).