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  • 产品名称：Lymphotoxin-alpha(LTA)(AA35-205)(Active)Protein

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  • 产品厂商：ACROBiosystems
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简单介绍：
Lymphotoxin-alpha(LTA)(AA35-205)(Active)Protein
详情介绍：
Brand ActiveMax®
Characteristics This protein carries no "tag". The protein has a calculated MW of 18.7 kDa. The protein migrates as 15 kDa and 19 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.5 ng/mL.
Background TNF-beta is also known as Lymphotoxin-alpha (LT-alpha or LTA), Tumor necrosis factor ligand superfamily member 1 (TNFSF1), which belongs to the tumor necrosis factor family. TNF-beta can form homotrimer with itself or form heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits. The homotrimer of TNF-beta is secreted and the heterotrimer is membrane-associated. The homotrimeric form of NF-beta binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR.
Molecular Weight 18.7 kDa
NCBI Accession NP_000586
UniProt P01374
Research Area Cytokines, Inflammation, Immunology
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Lymphotoxin-alpha (LTA) (AA 35-205) (Active) Protein (ABIN2181836) Human TNF-beta on SDS-PAGE under reducing (R) condition. The gel was stained overnigh...
Binding Studies (Bind) image for Lymphotoxin-alpha (LTA) (AA 35-205) (Active) Protein (ABIN2181836) Immobilized Human TNF-beta (Cat# TNB-H5214) at 5 μg/mL (100 µl/well), can bind Human...
Background publications Voigt, Maurer-Fogy, Adolf: "Natural human tumor necrosis factor beta (lymphotoxin). Variable O-glycosylation at Thr7, proteolytic processing, and allelic variation." in: FEBS letters, Vol. 314, Issue 1, pp. 85-8, 1992 (PubMed).

Gray, Aggarwal, Benton, Bringman, Henzel, Jarrett, Leung, Moffat, Ng, Svedersky: "Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity." in: Nature, Vol. 312, Issue 5996, pp. 721-4, 1985 (PubMed).