产品详情
查看大图

  • 产品名称：IPPIsomerase2(IDI2)(AA1-233)(Active)protein(Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
IPPIsomerase2(IDI2)(AA1-233)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.6 kDa. The protein migrates as 28 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Isopentenyl-diphosphate delta-isomerase 2 (IDI2) is also known as Isopentenyl pyrophosphate isomerase 2 (IPPI2 or IPP isomerase 2),which belongs to the IPP isomerase type 1 family and contains one nudix hydrolase domain. IDI2 catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).Recently, it indicates that, in humans, IDI2 is expressed only in skeletal muscle. Expression constructs of human IDI2 in Saccharomyces cerevisiae can complement isomerase function in an idi1-deficient yeast strain. Both isozymes, IDI1 and IDI2 are localized to the peroxisome by a PTS1-dependent pathway. IDI2 is regulated independently from IDI1, by a mechanism that may involve PPARalpha.
Molecular Weight 27.6 kDa
UniProt Q9BXS1
Research Area Cancer, Metabolism
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 150 mM NaCl, 50 mM Tris, pH 7.5 with 0.1 mM PMSF, 1 mM DTT
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for IPP Isomerase 2 (IDI2) (AA 1-233) (Active) protein (His tag) (ABIN2181247) Human IDI2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Clizbe, Owens, Masuda, Shackelford, Krisans: "IDI2, a second isopentenyl diphosphate isomerase in mammals." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6668-76, 2007 (PubMed).