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  • 产品名称：FolateReceptor2(Fetal)(FOLR2)(AA17-228)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
FolateReceptor2(Fetal)(FOLR2)(AA17-228)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. When Folic AcidBovine Serum Albumin was coated at 5 μg/mL (100 μL/well), the concentration of rhFOLR2 that produces 50% of the optimal binding response was found to be approximately 0.15-1.1 nM.

SDS-PAGE: resulting from glycosylation.
Background Folate receptor beta is also known as Folate receptor 2, FBP, FOLR2, BETA-HFR, FBP/PL-1, FR-BETA, FR-P3, and is a member of the folate receptor (FOLR) family. and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68 % and 79 % sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells. It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers. It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.
Molecular Weight 26 kDa
UniProt P14207
Research Area Signaling, Metabolism
Pathways Dicarboxylic Acid Transport
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Folate Receptor 2 (Fetal) (FOLR2) (AA 17-228) (Active) protein (His tag) (ABIN2181119) Folate Receptor 2 (Fetal) (FOLR2) (AA 17-228) (Active) protein (His tag)
Background publications van der Heijden, Oerlemans, Tak, Assaraf, Kraan, Scheffer, van der Laken, Lems, Scheper, Dijkmans, Jansen: "Involvement of breast cancer resistance protein expression on rheumatoid arthritis synovial tissue macrophages in resistance to methotrexate and leflunomide." in: Arthritis and rheumatism, Vol. 60, Issue 3, pp. 669-77, 2009 (PubMed).