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  • 产品名称：FolateReceptor1(Adult)(FOLR1)(AA25-233)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
FolateReceptor1(Adult)(FOLR1)(AA25-233)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.4 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. When Folic AcidBovine Serum Albumin is coated at 5 μg/mL (100 μL/well), the concentration of rhFOLR1 that produces 50% of the optimal binding response is found to be approximately 0.35-1.8 nM.

SDS-PAGE: resulting from glycosylation.
Background Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
Molecular Weight 25.4 kDa
NCBI Accession NP_057937
UniProt P15328
Pathways Dicarboxylic Acid Transport
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Folate Receptor 1 (Adult) (FOLR1) (AA 25-233) (Active) protein (His tag) (ABIN2181113) Folate Receptor 1 (Adult) (FOLR1) (AA 25-233) (Active) protein (His tag)
Background publications Lacey, Sanders, Rothberg, Anderson, Kamen: "Complementary DNA for the folate binding protein correctly predicts anchoring to the membrane by glycosyl-phosphatidylinositol." in: The Journal of clinical investigation, Vol. 84, Issue 2, pp. 715-20, 1989 (PubMed).