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  • 产品名称：beta-SiteAPP-CleavingEnzyme1(BACE)(AA22-457)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
beta-SiteAPP-CleavingEnzyme1(BACE)(AA22-457)(Active)protein(FcTag)
详情介绍：
Characteristics Human BACE-1, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 75 kDa. The predicted N-terminus is Glu. The reducing (R) protein migrates as 100-110 kDa in SDS-PAGE due to glycosylation.
Purity >85 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK (Dpn) RR-NH2, The specific activity is > 1.5 pmoles /min /μg.

SDS-PAGE: due to glycosylation.
Background Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD.
Molecular Weight 75 kDa
NCBI Accession NP_036236
UniProt P56817
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for beta-Site APP-Cleaving Enzyme 1 (BACE) (AA 22-457) (Active) protein (Fc Tag) (ABIN2180631) Human BACE-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Zacchetti, Chieregatti, Bettegazzi, Mihailovich, Sousa, Grohovaz, Meldolesi: "BACE1 expression and activity: relevance in Alzheimer's disease." in: Neuro-degenerative diseases, Vol. 4, Issue 2-3, pp. 117-26, 2007 (PubMed).

Willem, Garratt, Novak, Citron, Kaufmann, Rittger, DeStrooper, Saftig, Birchmeier, Haass: "Control of peripheral nerve myelination by the beta-secretase BACE1." in: Science (New York, N.Y.), Vol. 314, Issue 5799, pp. 664-6, 2006 (PubMed).