产品详情
查看大图

  • 产品名称：CD274Molecule(CD274)(AA19-238)(Active)protein(FcTag,AVItag,Biotin,Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CD274Molecule(CD274)(AA19-238)(Active)protein(FcTag,AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags. The protein has a calculated MW of 53.2 kDa. The protein migrates as 60-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human PDCD1,C-His Tag (Catalog # PD1-H5221) at 2 μg/mL can bind Biotinylated rh PD-L1 /B7-H1, C-His Tagged Fc.Avi Tag (Catalog # PD1-H82F3) with a linear range of 0.05-1 μg/mL . Measured by its binding ability in a functional ELISA. Immobilized human PDCD1,C-Fc Tag (Catalog # PD1-H5257) at 2 μg/mL can bind Biotinylated rh PD-L1 /B7-H1, C-His Tagged Fc.Avi Tag (Catalog # PD1-H82F3) with a linear range of 0.01-1 μg/mL .

SDS-PAGE: due to glycosylation. By co-expression with biotin ligase (BirA enzyme), Biotinylated rh PD-L1 /B7-H1, C-His Tagged Fc.Avi Tag was biotinylated in vivo for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Background Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.
Molecular Weight 53.2 kDa
NCBI Accession NP_054862
UniProt Q9NZQ7
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, 25 mM Arg, 10 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (Fc Tag,AVI tag,Biotin,His tag) (ABIN2181597) Biotinylated Human PD-L1, His Tag & Fc Tag on SDS-PAGE under reducing (R) condition. ...
Binding Studies (Bind) image for CD274 Molecule (CD274) (AA 19-238) (Active) protein (Fc Tag,AVI tag,Biotin,His tag) (ABIN2181597) Immobilized Human PD-1, Fc Tag with a linear range of 0.01-0.25 μg/mL.
Background publications Freeman, Long, Iwai, Bourque, Chernova, Nishimura, Fitz, Malenkovich, Okazaki, Byrne, Horton, Fouser, Carter, Ling, Bowman, Carreno, Collins, Wood, Honjo: "Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1027-34, 2000 (PubMed).

Dong, Zhu, Tamada, Chen: "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion." in: Nature medicine, Vol. 5, Issue 12, pp. 1365-9, 1999 (PubMed).