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  • 产品名称：High-MobilityGroupBox1(HMGB1)(AA1-215)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
High-MobilityGroupBox1(HMGB1)(AA1-215)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 28 kDa and 32 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA Immobilized human HMGB at 2 μg/mL (100 μL/well) can bind human AGER. The EC50 of human AGER is 0.22 μg/mL.
Background High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
Molecular Weight 25.7 kDa
NCBI Accession NP_002119
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for High-Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (His tag) (ABIN2181224) Human HMGB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Yang, Hreggvidsdottir, Palmblad, Wang, Ochani, Li, Lu, Chavan, Rosas-Ballina, Al-Abed, Akira, Bierhaus, Erlandsson-Harris, Andersson, Tracey: "A critical cysteine is required for HMGB1 binding to Toll-like receptor 4 and activation of macrophage cytokine release." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 26, pp. 11942-7, 2010 (PubMed).

Wang, Bloom, Zhang, Vishnubhakat, Ombrellino, Che, Frazier, Yang, Ivanova, Borovikova, Manogue, Faist, Abraham, Andersson, Andersson, Molina, Abumrad, Sama, Tracey: "HMG-1 as a late mediator of endotoxin lethality in mice." in: Science (New York, N.Y.), Vol. 285, Issue 5425, pp. 248-51, 1999 (PubMed).