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  • 产品名称：TissueFactorPathwayInhibitor(Lipoprotein-AssociatedCoagulationInhibitor)(TFPI)(

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  • 产品厂商：ACROBiosystems
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简单介绍：
TissueFactorPathwayInhibitor(Lipoprotein-AssociatedCoagulationInhibitor)(TFPI)(AA29-282)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2. The IC50 value is < 0.35 nM, as measured in 100 μL reaction mixture containing 1.25 ng trypsin, 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5.
Background Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF), The second K domain inhibits factor Xa, The third K domain binds to heparin, The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
Molecular Weight 30 kDa
NCBI Accession NP_006278
UniProt P10646
Research Area Cardiovascular
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-282) (Active) protein (His tag) (ABIN2181804) Human TFPI, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Bajaj, Mehta, Kashyap, Pushker, Lohia, Chawla, Ghose: "Clinical and pathologic profile of angiomyxomas of the orbit." in: Ophthalmic plastic and reconstructive surgery, Vol. 27, Issue 2, pp. 76-80, 2011 (PubMed).