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  • 产品名称：CD47(CD47)(AA19-139)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD47(CD47)(AA19-139)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh CD47 (Catalog # CD7-H5227) at 2 μg/mL can bind rh CD172a / SIRP-A Fc Chimera (Catalog # SIA-H5251) with a linear range of 0.5-25 ng/mL.

SDS-PAGE: due to glycosylation.
Background Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal ***** tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Molecular Weight 15.6 kDa
NCBI Accession NP_942088
UniProt Q08722
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD47 (CD47) (AA 19-139) (Active) protein (His tag) (ABIN2180804) Human CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Binding Studies (Bind) image for CD47 (CD47) (AA 19-139) (Active) protein (His tag) (ABIN2180804) Immobilized Human SIRP alpha, Fc Tag with a linear range of 1.56-12.5 ng/mL.
Background publications Piccio, Vermi, Boles, Fuchs, Strader, Facchetti, Cella, Colonna: "Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation." in: Blood, Vol. 105, Issue 6, pp. 2421-7, 2005 (PubMed).

Latour, Tanaka, Demeure, Mateo, Rubio, Brown, Maliszewski, Lindberg, Oldenborg, Ullrich, Delespesse, Sarfati et al.: "Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2547-54, 2001 (PubMed).

Lindberg, Gresham, Schwarz, Brown: "Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding." in: The Journal of cell biology, Vol. 123, Issue 2, pp. 485-96, 1993 (PubMed).