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  • 产品名称：Rabbitanti-Sheep(Ovine)IgG(FcRegion)Antibody

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  • 产品厂商：Acris-antibodies
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简单介绍：
Rabbitanti-Sheep(Ovine)IgG(FcRegion)Antibody
详情介绍：
Immunogen Purified normal IgG isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Purification Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Application Notes ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates to prepare conjugates of the user's own choice to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry Use: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000. Antibody titre : 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Restrictions For Research Use only
Buffer PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. Stabilizers: None
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months