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  • 产品名称:Rabbitanti-Cow(Bovine)IgG(WholeMolecule)Antibody(AlkalinePhosphatase(AP))

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  • 产品厂商:Acris-antibodies
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简单介绍:
Rabbitanti-Cow(Bovine)IgG(WholeMolecule)Antibody(AlkalinePhosphatase(AP))
详情介绍:
Immunogen Bovine IgG whole molecule.
Purification Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Background Synonyms: Bovine Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Bovine IgG in astandard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for thisproduct.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 with 0.01 % sodium azide as preservative and 10 mg/mL Polyethylene Glycol (PEG-8000) as stabilizer.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.