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  • 产品名称:Rabbitanti-Cat(Feline)IgG(FcRegion)Antibody(HRP)

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  • 产品厂商:Acris-antibodies
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简单介绍:
Rabbitanti-Cat(Feline)IgG(FcRegion)Antibody(HRP)
详情介绍:
Immunogen Cat IgG F(c) fragment
Purification Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Cat IgG, Cat Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes ELISA: 1/10000 - 1/50000. Western Blot: 1/1000 - 1/10000. Immunohistochemistry: 1/500 - 1/2500.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of deionized water (or equivalent)
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage -20 °C
Storage Comment Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-0 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).