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  • 产品名称：Mouseanti-HumanIgAAntibody

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  • 产品厂商：Acris-antibodies
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简单介绍：
Mouseanti-HumanIgAAntibody
详情介绍：
Clone DA43D3
Isotype IgG1
Purification Affinity Chromatography
Background Synonyms: Human Immunoglobulin A
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunohistochemistry on Frozen (1 μg/mL, 1/400) and Paraffin EmbeddedSections. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Protocol Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature

1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1 h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1 h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum.
Restrictions For Research Use only
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.4 mg/mL
Buffer PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use