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  • 产品名称：anti-CD40Ligandantibody(CD40LG)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD40Ligandantibody(CD40LG)(FITC)
详情介绍：
Immunogen Human CD154 transfected 300-19 cell (mouse pre-B cell line)
Clone 5F3
Isotype IgG1
Specificity This antibody reacts with CD154 antigen.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Protein A agarose
Alternative Name CD154 / CD40L (CD40LG Antibody Abstract)
Background The human CD154 (CD40-ligand) is a 39 kDa type II membrane glycoprotein predominantly expressed on CD4+ T cells, but also on a small proportion of CD8+ cells and NK cells. It is belonging to the tumor necrosis factor (TNF) family, while its counterreceptor on B cells, CD40, is a member of the TNF receptor/nerve growth factor (NGF) receptor family. Interactions between CD40 on B cells and its ligand on activated T cells results in B cell activation, proliferation, aggregation, and immunoglobulin isotype switching. A defect in the gene encoding CD154 is responsible for X-linked immunoglobulin deficiency with normal or elevated IgM (X-linked hyper IgM). These patients produce IgM but fail to produce downstream Ig isotypes, suggesting that interaction between CD40 on B cells and its ligand on activated T cells plays a critical role in Ig heavy chain switch recombination. The expression of CD154 on activated T cells is tightly regulated. B cells down-regulate the expression of functional CD154 in activated T cells through inhibition of CD154 mRNA expression and release of soluble CD40 that binds CD154.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5
Gene ID 959
NCBI Accession NP_000065
UniProt P29965
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer 10 mM NaPB ( pH 8.0), 0.15 M NaCl, 0.09 % NaN3, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Background publications Ishida, Kobayashi, Tojo, Ishida, Yamamoto, Inoue: "CD40 signaling-mediated induction of Bcl-XL, Cdk4, and Cdk6. Implication of their cooperation in selective B cell growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 12, pp. 5527-35, 1996 (PubMed).

Anand, Shapiro, Dickman, Prochownik: "Rhabdomyosarcomas do not contain mutations in the DNA binding domains of myogenic transcription factors." in: The Journal of clinical investigation, Vol. 93, Issue 1, pp. 5-9, 1994 (PubMed).

van Kooten, Gaillard, Galizzi, Hermann, Fossiez, Banchereau, Blanchard: "B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40." in: European journal of immunology, Vol. 24, Issue 4, pp. 787-92, 1994 (PubMed).

Nishioka, Lipsky: "The role of CD40-CD40 ligand interaction in human T cell-B cell collaboration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1027-36, 1994 (PubMed).

Tsubata, Wu, Honjo: "B-cell apoptosis induced by antigen receptor crosslinking is blocked by a T-cell signal through CD40." in: Nature, Vol. 364, Issue 6438, pp. 645-8, 1993 (PubMed).