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  • 产品名称：anti-EMR1antibody(Egf-LikeModuleContaining,Mucin-Like,HormoneReceptor-Like1)(Bi

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-EMR1antibody(Egf-LikeModuleContaining,Mucin-Like,HormoneReceptor-Like1)(Biotin)
详情介绍：
Immunogen Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Clone Cl:A3-1
Isotype IgG2b
Specificity This antibody recognizes the Murine F4/80 antigen, a 160 kDa cell surface glycoprotein. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5).
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Affinity Chromatography on Protein G.
Alternative Name Macrophage F4/80 Antigen (EMR1 Antibody Abstract)
Background F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
Gene ID 13733
NCBI Accession NP_034260
UniProt Q61549
Application Notes Flow Cytometry: Use 10 μL of neat to 1/10 diluted antibody to stain 10^6 cells in 100 μL. Immunohistology on Frozen and Paraffin Embedded Sections: For paraformaldehydeparaffin tissue, pretreatment by trypsinisation for 20-30 minutes is required. Use 1/50 oncryosections, 1/20 on paraffin sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Lin, Faunce, Stacey, Terajewicz, Nakamura, Zhang-Hoover, Kerley, Mucenski, Gordon, Stein-Streilein: "The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance." in: The Journal of experimental medicine, Vol. 201, Issue 10, pp. 1615-25, 2005 (PubMed).

Warschkau, Kiderlen: "A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3409-16, 1999 (PubMed).

Gordon, Lawson, Rabinowitz, Crocker, Morris, Perry: "Antigen markers of macrophage differentiation in murine tissues." in: Current topics in microbiology and immunology, Vol. 181, pp. 1-37, 1992 (PubMed).

Sunderkoetter, Beil, Roth, Sorg: "Cellular events associated with inflammatory angiogenesis in the mouse cornea." in: The American journal of pathology, Vol. 138, Issue 4, pp. 931-9, 1991 (PubMed).

Felix, Cecchini, Hofstetter, Elford, Stutzer, Fleisch: "Impairment of macrophage colony-stimulating factor production and lack of resident bone marrow macrophages in the osteopetrotic op/op mouse." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 5, Issue 7, pp. 781-9, 1990 (PubMed).

Kraal, Rep, Janse: "Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study." in: Scandinavian journal of immunology, Vol. 26, Issue 6, pp. 653-61, 1988 (PubMed).

Lee, Starkey, Gordon: "Quantitative analysis of total macrophage content in ***** mouse tissues. Immunochemical studies with monoclonal antibody F4/80." in: The Journal of experimental medicine, Vol. 161, Issue 3, pp. 475-89, 1985 (PubMed).

Hume, Perry, Gordon: "The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia." in: The Anatomical record, Vol. 210, Issue 3, pp. 503-12, 1985 (PubMed).

Hume, Robinson, MacPherson, Gordon: "The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs." in: The Journal of experimental medicine, Vol. 158, Issue 5, pp. 1522-36, 1983 (PubMed).

Austyn, Gordon: "F4/80, a monoclonal antibody directed specifically against the mouse macrophage." in: European journal of immunology, Vol. 11, Issue 10, pp. 805-15, 1982 (PubMed).