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  • 产品名称：anti-CD3deltaChainantibody(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD3deltaChainantibody(FITC)
详情介绍：
Immunogen F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.
Clone 1F4
Isotype IgM
Specificity SM253F recognises rat CD3, a 25 kDa antigen which is found on rat T cells. SM253F does not react with rat B cells.
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: CD3D, T-cell surface glycoprotein CD3 delta chain, T3D
Gene ID 25710
NCBI Accession NP_037301
UniProt P19377
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T- cells in the presence of PMA (2). We recommend antibody ABIN120367 for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer.
Background publications Nicolls, Aversa, Pearce, Spinelli, Berger, Gurley, Hall: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." in: Transplantation, Vol. 55, Issue 3, pp. 459-68, 1993 (PubMed).

Tanaka, Masuko, Yagita, Tamura, Hashimoto: "Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2791-5, 1989 (PubMed).