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  • 产品名称：anti-CD163antibody(CD163Molecule)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD163antibody(CD163Molecule)(FITC)
详情介绍：
Immunogen Mononuclear splenocytes isolated from a normal donor after a traumatic rupture.
Clone Ber-Mac3
Isotype IgG1
Specificity This antibody reacts with CD163 antigen on Flow Cytometry.
Cross-Reactivity (Details) Species reactivity (expected):Monkey.
Species reactivity (tested):Human.
Characteristics Synonyms: M130, Hemoglobin scavenger receptor, Macrophage marker, Scavenger receptorcysteine-rich type 1 protein M130
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Alternative Name CD163 (CD163 Antibody Abstract)
Background CD163, also known as M130, Ber-Mac3, Ki-M8 or SM4, is a 130-150 kDa member of the scavenger receptor cysteine-rich (SRCR) superfamily. CD163 scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 expression is restricted to cells of monocyte lineage and increases as monocytes mature into macrophages. CD163 is up-regulated on mononuclear phagocytes by IL-10, IL-6, and dexamethasone, while lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) induce shedding of CD163 from the cell surface. Several CD163 isoforms exist, which differ in their cytoplasmic domains and putative phosphorylation sites.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
Gene ID 9332
UniProt Q86VB7
Research Area Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: 5-10 μg/mL (final concentration). Positive Control: Monocytes. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Flow cytometric analysis for floating cellsProtocol 1. We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS with 2% FCS and 0. 1% NaN]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atRT (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well and incubate for 5 minutes at RT. 5) Add 20 µL of the FITC labeled CD163 monoclonalantibody (Ber-Mac3) (5-10 µg/mL) diluted with thewashing buffer. Mix well and incubate for 30 minutes at RT (20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: MonocytesProtocol 2. We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBScontaining 2% fetal calf serum (FCS) and 0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the FITC labeled CD163 monoclonal antibody (Ber-Mac3) (50 µg/ml) dilutedwith the washing buffer into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well and incubate for 30 minutes atRT (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all step described below. 1) Add 20 µL of the FITC labeled CD163 monoclonal antibody (Ber-Mac3) (5-10 µg/mL)diluted with the washing buffer [PBS containing 2% fetal calf serum (FCS) and 0. 1% NaN3]into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at RT(20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Add 1 mL of H2O to each tube and incubate for 10 minutes at RT (20~25°C). 5) Centrifuge at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by carefulaspiration. 6) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
Restrictions For Research Use only
Concentration 50 µg/mL
Buffer PBS containing 1 % BSA and 0.09 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-CD163 antibody (CD163 Molecule) (FITC) (ABIN492602) anti-CD163 Molecule (CD163) antibody (FITC)