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  • 产品名称：anti-CD45antibody(ProteintyrosinePhosphatase,ReceptorType,C)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD45antibody(ProteintyrosinePhosphatase,ReceptorType,C)(FITC)
详情介绍：
Immunogen Purified Rat spleen L-CA. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Clone OX-33
Isotype IgG1
Specificity This antibody is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes.
Purification Affinity Chromatography on Protein G.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. this antibody does not always give a clear separation between positives and negatives in some cell preparations, if users find this to be the case we recommend the use of R. Phycoerythrin conjugated reagent ABIN120481 which generally exhibits a peak of higher mean fluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Barclay, Jackson, Willis, Williams: "Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus." in: The EMBO journal, Vol. 6, Issue 5, pp. 1259-64, 1987 (PubMed).

Woollett, Barclay, Puklavec, Williams: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).