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  • 产品名称：anti-ITGALantibody(Integrinalpha-L)(Biotin)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-ITGALantibody(Integrinalpha-L)(Biotin)
详情介绍：
Immunogen Leukocytes from a pacient suffering from a LGL-type leukaemia.
Clone MEM-25
Isotype IgG1
Specificity The antibody MEM-25 reacts with CD11a (α-subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Alternative Name CD11a / ITGAL (ITGAL Antibody Abstract)
Background CD11a (LFA-1α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain
Gene ID 3683
NCBI Accession NP_002200
UniProt P20701
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Activated T Cell Proliferation, Integrin Complex
Application Notes This biotynated antibody is suitable for indirect Immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1/120.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 15 mM sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Product cited in: Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Background publications Fujii, Takada-Takatori, Kawashima: "Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity." in: Life sciences, Vol. 80, Issue 24-25, pp. 2320-4, 2007 (PubMed).

Giblin, Lemieux: "LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics." in: Current pharmaceutical design, Vol. 12, Issue 22, pp. 2771-95, 2006 (PubMed).

Kellersch, Kolanus: "Membrane-proximal signaling events in beta-2 integrin activation." in: Results and problems in cell differentiation, Vol. 43, pp. 245-57, 2006 (PubMed).

Sarantos, Raychaudhuri, Lum, Staunton, Simon: "Leukocyte function-associated antigen 1-mediated adhesion stability is dynamically regulated through affinity and valency during bond formation with intercellular adhesion molecule-1." in: The Journal of biological chemistry, Vol. 280, Issue 31, pp. 28290-8, 2005 (PubMed).

Mathison, Befus, Davison, Woodman: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Hajishengallis, Martin, Sojar, Sharma, Schifferle, DeNardin, Russell, Genco: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Ottonello, Epstein, Dapino, Barbera, Morone, Dallegri et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b ..." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

Simon, Cherapanov, Nadra, Waddell, Seo, Wang, Doerschuk, Downey: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).