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  • 产品名称：anti-CD8antibody(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD8antibody(FITC)
详情介绍：
Immunogen Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Clone OX-8
Isotype IgG1
Specificity This antibody recognises the CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies.
Purification Ion Exchange Chromatography
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 24930
NCBI Accession NP_113726
UniProt P07725
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Popov, Dela Cruz, Barber, Chiu, Inman: "The effect of an anti-HLA-B27 immune response on CTL recognition of Chlamydia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3375-82, 2001 (PubMed).

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985 (PubMed).

Bukovský, Presl, Zidovský: "Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy." in: Immunology, Vol. 52, Issue 4, pp. 631-40, 1984 (PubMed).

Cantrell, Robins, Brooks, Baldwin: "Phenotype of rat natural killer cells defined by monoclonal antibodies marking rat lymphocyte subsets." in: Immunology, Vol. 45, Issue 1, pp. 97-103, 1982 (PubMed).

Dallman, Mason, Webb: "The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells." in: European journal of immunology, Vol. 12, Issue 6, pp. 511-8, 1982 (PubMed).

Lyscom, Brueton: "Intraepithelial, lamina propria and Peyer's patch lymphocytes of the rat small intestine: isolation and characterization in terms of immunoglobulin markers and receptors for monoclonal antibodies." in: Immunology, Vol. 45, Issue 4, pp. 775-83, 1982 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980 (PubMed).